Gamborg’s B-5 Medium Powder Yield 3000 ml (+Hormones, +Sucrose, -Agar)

Description

Item:
Gamborg’s B-5 Medium Powder Yield 3000 ml (+Hormones,+Sucrose, -Agar)
Plant hormones/regulators and sucrose are included in this listing and packed separately.
Flexible for users to prepare medium with desired concentrations of hormones and sugar.
This product does not include agar or other gelling agents, users need to add agar if pouring agar nutrient gel.
Details:
This listing product includes 4 items, packed separately as shown in listing photos:
9.8 grams Gamborg’s B-5 Medium Dehydrate Powder, to make 3000 ml medium (3 Liters or 0.77 gallon).
100 mg (0.1g) BA (6-BA or 6-BAP, or 6-Benzylaminopurine), plant hormone/growth regulator, packed separately, commonly used concentration 1 mg/L.
100 mg (0.1g) NAA (Sodium Alpha-Naphthaleneacetic Acid), plant hormone/growth regulator, packed separately, commonly used concentration 0.1 mg/L.
15 grams sucrose, packed separately, commonly used concentration 0.5 -5%.
Uses and Storage:
For
in vitro
plant cell line and tissue culture
For cloning and growing plantlets
Long-term storage: sealed tightly, wrapped with foil and stored in refrigerator (4-10°C )
Shipping:
Fast shipping US domestic via USPS First Class Mail and globally via eBay International Shipping Services
Other Information:
Some opinions on plant tissue/cell culture:
Murashige and Skoog (MS) medium is a good start, but culture media depend a lots on the plants you're working on; check the literature for previous work.
MS medium is quite good for induction of non-organized growth (callus), but may prevent plant organogenesis.
Half strength MS (MS/2), Gamborg’s B-5 Medium and Half Gamborg’s B-5 Medium may be the alternatives to reduce plant vitrification/hyperhydricity.
Microbial contamination is still the top issue in plant tissue/cell culture for both amateur and professional.
A decrease of sucrose concentration and an increase of CO
2
supply may reduce fungal/microbial contamination.
Consider co-culture of plant tissue/cell with its naturally associated microorganisms if stubborn contamination occurs.
Check our contamination inhibitors SHY and TICO, natural plant/microorganism extracts.